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Biblioteca(s): |
Embrapa Agricultura Digital. |
Data corrente: |
14/02/2013 |
Data da última atualização: |
08/01/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BITAR, M.; DRUMMOND, M. G.; COSTA, M. G. S.; LOBO, F. P.; CALZAVARA-SILVA, C. E.; BISCH, P. M.; MACHADO, C. R.; MACEDO, A. M.; PIERCE, R. J.; FRANCO, G. R. |
Afiliação: |
MAINÁ BITAR, UFRJ, UFMG; MARCELA GONÇALVES DRUMMOND, UFMG, Univ. Lille Nord de France; MAURICIO GARCIA SOUZA COSTA, UFRJ; FRANCISCO PEREIRA LOBO, CNPTIA; CARLOS EDUARDO CALZAVARA-SILVA, Fiocruz; PAULO MASCARELLO BISCH, UFRJ; CARLOS RENATO MACHADO, UFMG; ANDRÉA MARA MACEDO, UFMG; RAYMOND J. PIERCE, Univ. Lille Nord de France; GLÓRIA REGINA FRANCO, UFMG. |
Título: |
Modeling the zing finger protein SmZF1 from Schistosoma mansoni: Insights into DNA binding and gene regulation. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Journal of Molecular Graphics and Modelling, New York, v. 39, p. 29-38, 2013. |
Idioma: |
Inglês |
Conteúdo: |
Zinc finger proteins are widely found in eukaryotes, representing an important class of DNA-binding proteins frequently involved in transcriptional regulation. Zinc finger motifs are composed by two antiparallel b-strands and one a-helix, stabilized by a zinc ion coordinated by conserved histidine and cysteine residues. In Schistosoma mansoni, these regulatory proteins are known to modulate morphological and physiological changes, having crucial roles in parasite development. A previously described C2H2 zinc finger protein, SmZF1, was shown to be present in cell nuclei of different life stages of S. mansoni and to activate gene transcription in a heterologous system. A high-quality SmZF1 tridimensional structure was generated using comparative modeling. Molecular dynamics simulations of the obtained structure revealed stability of the zinc fingers motifs and high flexibility on the terminals, comparable to the profile observed on the template X-ray structure based on thermal b-factors. Based on the protein tridimensional features and amino acid composition, we were able to characterize four C2 H2 zinc finger motifs, the first involved in protein?protein interactions while the three others involved in DNA binding. We defined a consensus DNA binding sequence using three distinct algorithms and further carried out docking calculations, which revealed the interaction of fingers 2?4 with the predicted DNA. A search for S. mansoni genes presenting putative SmZF1 binding sites revealed 415 genes hypothetically under SmZF1 control. Using an automatic annotation and GO assignment approach, we found that the majority of those genes code for proteins involved in developmental processes. Taken together, these results present a consistent base to the structural and functional characterization of SmZF1. MenosZinc finger proteins are widely found in eukaryotes, representing an important class of DNA-binding proteins frequently involved in transcriptional regulation. Zinc finger motifs are composed by two antiparallel b-strands and one a-helix, stabilized by a zinc ion coordinated by conserved histidine and cysteine residues. In Schistosoma mansoni, these regulatory proteins are known to modulate morphological and physiological changes, having crucial roles in parasite development. A previously described C2H2 zinc finger protein, SmZF1, was shown to be present in cell nuclei of different life stages of S. mansoni and to activate gene transcription in a heterologous system. A high-quality SmZF1 tridimensional structure was generated using comparative modeling. Molecular dynamics simulations of the obtained structure revealed stability of the zinc fingers motifs and high flexibility on the terminals, comparable to the profile observed on the template X-ray structure based on thermal b-factors. Based on the protein tridimensional features and amino acid composition, we were able to characterize four C2 H2 zinc finger motifs, the first involved in protein?protein interactions while the three others involved in DNA binding. We defined a consensus DNA binding sequence using three distinct algorithms and further carried out docking calculations, which revealed the interaction of fingers 2?4 with the predicted DNA. A search for S. mansoni genes presenting putative SmZF1 binding sites reve... Mostrar Tudo |
Palavras-Chave: |
Ligação de DNA; Regulação gênica. |
Thesagro: |
Schistosoma Mansoni. |
Thesaurus Nal: |
DNA-binding proteins; Gene expression regulation; Zinc finger motif. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02751naa a2200301 a 4500 001 1949183 005 2020-01-08 008 2013 bl uuuu u00u1 u #d 100 1 $aBITAR, M. 245 $aModeling the zing finger protein SmZF1 from Schistosoma mansoni$bInsights into DNA binding and gene regulation.$h[electronic resource] 260 $c2013 520 $aZinc finger proteins are widely found in eukaryotes, representing an important class of DNA-binding proteins frequently involved in transcriptional regulation. Zinc finger motifs are composed by two antiparallel b-strands and one a-helix, stabilized by a zinc ion coordinated by conserved histidine and cysteine residues. In Schistosoma mansoni, these regulatory proteins are known to modulate morphological and physiological changes, having crucial roles in parasite development. A previously described C2H2 zinc finger protein, SmZF1, was shown to be present in cell nuclei of different life stages of S. mansoni and to activate gene transcription in a heterologous system. A high-quality SmZF1 tridimensional structure was generated using comparative modeling. Molecular dynamics simulations of the obtained structure revealed stability of the zinc fingers motifs and high flexibility on the terminals, comparable to the profile observed on the template X-ray structure based on thermal b-factors. Based on the protein tridimensional features and amino acid composition, we were able to characterize four C2 H2 zinc finger motifs, the first involved in protein?protein interactions while the three others involved in DNA binding. We defined a consensus DNA binding sequence using three distinct algorithms and further carried out docking calculations, which revealed the interaction of fingers 2?4 with the predicted DNA. A search for S. mansoni genes presenting putative SmZF1 binding sites revealed 415 genes hypothetically under SmZF1 control. Using an automatic annotation and GO assignment approach, we found that the majority of those genes code for proteins involved in developmental processes. Taken together, these results present a consistent base to the structural and functional characterization of SmZF1. 650 $aDNA-binding proteins 650 $aGene expression regulation 650 $aZinc finger motif 650 $aSchistosoma Mansoni 653 $aLigação de DNA 653 $aRegulação gênica 700 1 $aDRUMMOND, M. G. 700 1 $aCOSTA, M. G. S. 700 1 $aLOBO, F. P. 700 1 $aCALZAVARA-SILVA, C. E. 700 1 $aBISCH, P. M. 700 1 $aMACHADO, C. R. 700 1 $aMACEDO, A. M. 700 1 $aPIERCE, R. J. 700 1 $aFRANCO, G. R. 773 $tJournal of Molecular Graphics and Modelling, New York$gv. 39, p. 29-38, 2013.
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Embrapa Agricultura Digital (CNPTIA) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
04/02/2008 |
Data da última atualização: |
04/02/2008 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Nacional - B |
Autoria: |
VIEIRA, E. A.; CARVALHO, F. I. de; OLIVEIRA, A. C. de; MARTINS, L. F.; BENIN, G.; SILVA, J. A. G. da; COIMBRA, J.; MARTINS, A. F. : CARVALHO, M. F. de; RIBEIRO, G. |
Afiliação: |
Eduardo Alano Vieira, CPAC; Fernando Irajá Félix de Carvalho, UFPel; Antonio Costa de Oliveira, UFPel; Luís Fernando Martins, UFPel; Giovani Benin, UTFPR; José Antônio Gonzalez da Silva, UFPel; Jéferson Coimbra, UFPel; Andreza Figueirola Martins, UFPel. |
Título: |
Análise de trilha entre os componentes primários e secundários do rendimento de grãos em trigo. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
Revista Brasileira de Agrociência, Pelotas, v. 13, n. 2, p. 169-174, abr./jun. 2007. |
Idioma: |
Português |
Palavras-Chave: |
Correlação linear. |
Thesagro: |
Melhoramento Vegetal; Trigo; Triticum Aestivum. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00790naa a2200253 a 4500 001 1559261 005 2008-02-04 008 2007 bl uuuu u00u1 u #d 100 1 $aVIEIRA, E. A. 245 $aAnálise de trilha entre os componentes primários e secundários do rendimento de grãos em trigo. 260 $c2007 650 $aMelhoramento Vegetal 650 $aTrigo 650 $aTriticum Aestivum 653 $aCorrelação linear 700 1 $aCARVALHO, F. I. de 700 1 $aOLIVEIRA, A. C. de 700 1 $aMARTINS, L. F. 700 1 $aBENIN, G. 700 1 $aSILVA, J. A. G. da 700 1 $aCOIMBRA, J. 700 1 $aMARTINS, A. F. : CARVALHO, M. F. de 700 1 $aRIBEIRO, G. 773 $tRevista Brasileira de Agrociência, Pelotas$gv. 13, n. 2, p. 169-174, abr./jun. 2007.
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